gotop
首頁 免疫新知

關節炎臨床研究

膝關節疼痛綜合症患者:膝關節脂肪墊中IL-6和TNF-α表現的研究報告
2019/01/20

ncreased interleukin 6 and tumour necrosis factor α expression in the infrapatellar fat pad of the knee joint with the anterior knee pain syndrome: a preliminary report.

Witoński D1Wągrowska-Danilewicz MKęska RRaczyńska-Witońska GStasikowska-Kanicka O.

Abstract

The pathway of pain in the anterior knee pain syndrome remains unclear. It has been hypothesized that some biochemical mediators of inflammation, such as cytokines contribute to the process. The objective of this work was to evaluate the synovial membrane and the infrapatellar fat pad expression of the inflammatory mediators and potentially chondrodestructive cytokines interleukin 6 (IL-6) and tumour necrosis factor α (TNF-α) in the anterior knee pain syndrome, and to determine whether the cytokine expression counterpart with/corresponds to the amount of chondral damage in this syndrome. Ten consecutive patients with the anterior knee pain syndrome (group I) participated in the study. Patients with a history of trauma were excluded from this group. For comparison we used 10 patients with anterior cruciate ligament rupture or meniscal lesion with no history of pain in the anterior compartment (group II). Immunohistochemical techniques using a polyclonal rabbit anti-human antibody to IL-6 and a monoclonal mouse anti-human antibody to TNF-α were employed. The results show a statistically significant higher expression of IL-6 in infrapatellar fat pad (p < 0.05) as well as TNF-α in the infrapatellar fad pad and the synovium (p < 0.03, and p < 0.02, respectively) in group I as compared to control subjects. There is no any difference in the amount of chondral damage present in group I as compared to group II. The results of this study provide the immunohistochemical evidence suggesting that the anterior knee pain syndrome could be characterized by infrapatellar fat pad and synovial inflammation variations without the articular cartilage loss.

PMID: 21290344

https://www.ncbi.nlm.nih.gov/pubmed/21290344

翻譯:

膝關節疼痛綜合症患者:膝關節脂肪墊中IL-6與TNF-α表現的研究報告

作者:Witoński D1Wągrowska-Danilewicz MKęska RRaczyńska-Witońska GStasikowska-Kanicka O.

摘要:

前膝關節疼痛綜合症的疼痛途徑尚不清楚,據推測,發炎症狀來自於一些生物化學介質,如:細胞激素促成發炎過程。這項研究的目的是評估前膝關節疼痛綜合症患者,炎性介質和潛在的軟骨破壞性因子IL-6和TNF-α在滑膜及髕下脂肪墊的表現。以確定細胞激素對應物在該綜合症中軟骨損傷的量。

10位前關節疼痛綜合症患者參加了這項研究。有創傷史的患者則排除在這個研究之外。為了進行比較,我們採用了前交叉韌帶斷裂或前關節腔內沒有疼痛史的半月板損傷的10名患者。

免疫組織化學技術採用多克隆兔抗人抗體的IL-6以及單克隆小鼠抗人抗體的TNF-α。結果顯示,髕下脂肪墊中IL-6的表現(p<0.05)以及髕骨上脂肪墊和滑膜中的TNF-α(分別為p<0.03和p<0.02)與對照受試者相比。兩組軟骨損傷量沒有任何差異。

這項研究的結果提供免疫組織化學證據表明,前膝關節疼痛綜合症的問題,可能是髕下脂肪墊和滑膜炎症的變化,沒有關節軟骨損失。

PMID: 21290344

https://www.ncbi.nlm.nih.gov/pubmed/21290344